BRINE SHRIMP CYTOTOXIC ACTIVITY OF 50% AQUEOUS ETHANOLIC LEAF EXTRACT OF CALOTROPIS PROCERA R. BR

Objective: The objective of this work entails a preliminary screening of 50% aqueous ethanolic leaf extract of Calotropis procera for locating antitumoractivity.Methods: 50% aqueous ethanolic extract, obtained from dried powdered plant material of C. procera was partitioned sequentially in petroleumether, benzene, and chloroform. Each fraction thus obtained was concentrated in a vacuum evaporator. The residual mass was collected separatelyand dissolved in propylene glycol. The three samples were subjected to brine shrimp cytotoxic assay to locate if there may be any positive responseof antitumor activity.Result: It was found that the sample obtained from chloroform extract responded positively in brine shrimp test and showed lethal concentration(LC50 at which 50% individual dies) at the concentration of 5 mg/ml. Benzene extract showed LC at the concentration of 15 mg/ml and petroleumether showed LC50 at the concentration of 20 mg/ml.Conclusion: Proves the presence of antitumor phytochemicals in C. procera.Keywords: Antitumor compounds, Brine shrimp, Calotropis procera, Cytotoxic activity

Microbiological examination of milk in Tarakeswar, India with special reference to coliforms

A study was carried out to assess the milk quality in Tarakeswar, India with special reference to coliforms. By standard plate count (SPC) method, out of ten raw milk samples, the microbial colonies were found to be high in six samples and the colony content was low in rest four samples. Inpasteurized milk samples, the colonies were low in seven samples and high in three samples. The methylene blue test performed for raw milk samples showed that out of ten samples, the five samples were poor, two samples were fair, two samples were good and only one sample was found to be anexcellent. Out of ten pasteurized samples, nine samples were of good quality and one was found to be excellent. Bacterial colony was found to be opaque and metallic sheen in colour prepared from five raw milk samples, and by biochemical characterization it was identified as Escherichia col

The visibility based Tapered Gridded Estimator (TGE) for the redshifted 21-cm power spectrum

We present the improved visibility based Tapered Gridded Estimator (TGE) for the power spectrum of the diffuse sky signal. The visibilities are gridded to reduce the computation, and tapered through a convolution to suppress the contribution from the outer regions of the telescope's field of view. The TGE also internally estimates the noise bias, and subtracts this out to give an unbiased estimate of the power spectrum. An earlier version of the 2D TGE for the angular power spectrum $C_{\ell}$ is improved and then extended to obtain the 3D TGE for the power spectrum $P({\bf k})$ of the 21-cm brightness temperature fluctuations. Analytic formulas are also presented for predicting the variance of the binned power spectrum. The estimator and its variance predictions are validated using simulations of $150 \, {\rm MHz}$ GMRT observations. We find that the estimator accurately recovers the input model for the 1D Spherical Power Spectrum $P(k)$ and the 2D Cylindrical Power Spectrum $P(k_\perp,k_\parallel)$, and the predicted variance is also in reasonably good agreement with the simulations.Comment: 19 pages, 13 figures. Accepted for publication in MNRAS. The definitive version will be available at http://mnrasl.oxfordjournals.org

Biotechnological potential of natural food grade biocolorants

Color becomes the most sensitive part of any commodity not only for its appeal but also it enhances consumer acceptability. In addition, the color of a food substance is important to indicate its freshness and safety that are also indices of good aesthetic and sensorial values. For natural color and additives, adherence to the norms of biosafety protocol, are limited. The demand for natural source of such compounds is increasing day by day because of awareness of positive health benefit out of natural compounds. It therefore, necessitates looking into natural sources of food grade colorants and their use potentials. It is found more justified to use the term biocolorant instead of biopigment. Since pigments are mostly water insoluble with exceptions of certain pigments of biological origin. This article includes the advancements of process development and other biotechnological aspects of natural food grade colorants

Integrated Parametric Graph Closure and Branch-and-Cut Algorithm for Open Pit Mine Scheduling under Uncertainty

Open pit mine production scheduling is a computationally expensive large-scale mixed-integer linear programming problem. This research develops a computationally efficient algorithm to solve open pit production scheduling problems under uncertain geological parameters. The proposed solution approach for production scheduling is a two-stage process. The stochastic production scheduling problem is iteratively solved in the first stage after relaxing resource constraints using a parametric graph closure algorithm. Finally, the branch-and-cut algorithm is applied to respect the resource constraints, which might be violated during the first stage of the algorithm. Six small-scale production scheduling problems from iron and copper mines were used to validate the proposed stochastic production scheduling model. The results demonstrated that the proposed method could significantly improve the computational time with a reasonable optimality gap (the maximum gap is 4%). In addition, the proposed stochastic method is tested using industrial-scale copper data and compared with its deterministic model. The results show that the net present value for the stochastic model improved by 6% compared to the deterministic model

Effect of dust and anthropogenic aerosols on columnar aerosol optical properties over Darjeeling (2200 m asl), Eastern Himalayas, India

Background: The loading of atmospheric particulate matter (aerosol) in the eastern Himalaya is mainly regulated by the locally generated anthropogenic aerosols from the biomass burning and by the aerosols transported from the distance sources. These different types of aerosol loading not only affect the aerosol chemistry but also produce consequent signature on the radiative properties of aerosol. Methodology/Principal Findings: An extensive study has been made to study the seasonal variations in aerosol components of fine and coarse mode aerosols and black carbon along with the simultaneous measurements of aerosol optical depth on clear sky days over Darjeeling, a high altitude station (2200 masl) at eastern Himalayas during the year 2008. We observed a heavy loading of fine mode dust component (Ca2+) during pre-monsoon (Apr - May) which was higher by 162 than its annual mean whereas during winter (Dec - Feb), the loading of anthropogenic aerosol components mainly from biomass burning (fine mode SO4 2- and black carbon) were higher (76 for black carbon and 96 for fine mode SO4 2-) from their annual means. These high increases in dust aerosols during pre-monsoon and anthropogenic aerosols during winter enhanced the aerosol optical depth by 25 and 40, respectively. We observed that for every 1 increase in anthropogenic aerosols, AOD increased by 0.55 during winter whereas for every 1 increase in dust aerosols, AOD increased by 0.46 during pre-monsoon. Conclusion/Significance: The natural dust transport process (during pre-monsoon) plays as important a role in the radiation effects as the anthropogenic biomass burning (during winter) and their differential effects (rate of increase of the AOD with that of the aerosol concentration) are also very similar. This should be taken into account in proper modeling of the atmospheric environment over eastern Himalayas

Exploring strategies for classification of external stimuli using statistical features of the plant electrical response

This is the author accepted manuscript. The final version is available from the Royal Society via the DOI in this record.Plants sense their environment by producing electrical signals which in essence represent changes in underlying physiological processes. These electrical signals, when monitored, show both stochastic and deterministic dynamics. In this paper, we compute 11 statistical features from the raw non-stationary plant electrical signal time series to classify the stimulus applied (causing the electrical signal). By using different discriminant analysis-based classification techniques, we successfully establish that there is enough information in the raw electrical signal to classify the stimuli. In the process, we also propose two standard features which consistently give good classification results for three types of stimuli--sodium chloride (NaCl), sulfuric acid (H₂SO₄) and ozone (O₃). This may facilitate reduction in the complexity involved in computing all the features for online classification of similar external stimuli in future.The work reported in this paper was supported by project PLants Employed As SEnsor Devices (PLEASED), EC grant agreement number 296582

The cost and effective analysis of health care management of very low birth weight babies in rural areas of West Bengal, India

Background: Low birth weight (LBW) is prevalent in low-income countries. Level II neonatal intensive care at SCNUs is cost intensive. Rational use of SCNU services by targeting its utilization for the VLBW neonates and maintenance of community based newborn care is required.  Even though the economic evaluation of interventions to reduce this burden is essential to guide health care policy making for low resource setting, data on low cost outcome study associated with LBW in Indian setup are scarce.  Methods: This study aims to estimate the costs to the health system in the management of LBW in rural setting where affordability of parents for healthcare facility comparatively less. The cost of management was cut off by minimum investigations, more supervision by working health care personnel’s. A prospective observational study was conducted to see outcome of estimate the costs to the health system in the management of LBW or VLBW babies.Results: The mortality and survival rate among the evaluated LBW under this low cost health care setting was 6.66% and 86.6% respectively.Conclusions: Results of this unique cost and effectiveness evaluation of LBW healthcare management in a low resource setting are very relevant in Indian context where healthcare facility is almost out of reach and affordability in majority rural populations. These results are of relevance for similar settings and should serve to promote interventions aimed at improving maternal care in rural settings. Further larger research is required on cost effectiveness of level II neonatal intensive care.

Altered resistin and IL6 in Neonatal sepsis in patients admitted in a tertiary care teaching hospital at Eastern India

Background: Neonatal sepsis is a clinical syndrome followed by bacteraemia in the first month of life and appears to be one of the primary causes of mortality and morbidity worldwide. The study aim was to detect the levels of resistin, IL-6, CRP and hs-CRP in clinically suspected cases of neonatal sepsis and establish its association with the pathogenesis of the disease. Materials & Methods: The case control study consists of 78 neonates of whom 42 were clinically suspected case of sepsis admitted in NICU of Paediatric department and were taken as cases and 36 were normal healthy neonates taken as control subjects in a tertiary care teaching hospital, Durgapur, West Bengal. The cases as well as controls were within 28 days of age. Preterm and term neonates (< 28 days of age) of both sexes showing signs of both early and late onset sepsis and also blood culture positive were included in the study. Two ml of blood was collected without anticoagulant and serum was separated by centrifugation at 3500 rpm for 15-20 mins and was used for measurement of hs-CRP, resistin and IL 6. Serum hs-CRP levels was determined with a high-sensitivity nephelometric method while the serum level of IL-6 and Resistin were measured by immunoassay Kits (Raybiotech, USA). Results: Serum resistin levels were increased in sepsis cases as compared to controls and were statistically significant (38.96 ± 17.15 vs 15.49 ± 8.54 ng/ml; p < 0.0001). It was also observed that serum IL 6 levels were higher in sepsis cases as compared to controls which was statistically significant (58.19 ± 39.97 versus 8.48 ± 3.90 pg/ml; P < 0.0001). However, a weak positive correlation was observed between serum resistin with serum IL 6 level (r = 0.343; P = 0.025) among neonatal sepsis subjects while no correlation was seen in controls (r = 0.141; P = 0.411). Conclusion: The measurement of these sepsis markers is extremely important only in case of neonates with unclear infectious status. We have observed a significant rise in Resistin or IL 6 or hs-CRP which may be suggested as specific marker for the identification of neonatal sepsis.  The combination of Resistin or IL 6 or CRP or hs-CRP could therefore be crucial for the diagnosis and would be better predictors of neonatal sepsis and may be crucial in the pathogenesis of the disease. Keywords: Preterm neonates, neonatal sepsis, mortality and morbidity, serum resistin, interleukin-6 (IL-6), hs-CR

Knowledge, attitudes and practice of adverse drug reaction monitoring among physicians in India

Background: Underreporting of ADRs still remains a major obstacle in the complete success of pharmacovigilance programs. In order to improve ADR monitoring, it is thus imperative to assess the current knowledge, attitude, and practices of doctors. Therefore, the primary objective of this study was to evaluate the knowledge, attitude, and practices (KAP) of the healthcare professionals about pharmacovigilance in various tertiary care government teaching hospital vis-a-vis private clinics in West Bengal.Methods: A cross sectional, questionnaire based survey was conducted among healthcare practitioners in several tertiary care government set-ups and private set-ups in the state of West Bengal (India). The study instrument was a pre-validated structured questionnaire designed to obtain information on the knowledge of the ADRs reporting, the attitudes towards the reporting, and the factors that in practice could hinder the reporting among the doctors.Results: About 89.62% public practitioners correctly spotted the WHO definition for pharmacovigilance, while 77.5% of the private practitioners did the same. Only 19.81% of the public practitioners documented a suspected ADR in any surveillance form, while there were only 3.75% private practitioners who documented it. About 59.43% of the physicians in government hospitals published an ADR case report in any medical journal, while 81.25% private practitioners did no.Conclusions: Study revealed lack of time, incentive less extra work load being major factors responsible for ADR underreporting. In order to improve ADR reporting, continuous medical education, training and proper sensitization of healthcare professionals can help combating the existing scenario and promising an improved tomorrow. The PvPI should be widely publicized in the visual and print media to make health professionals, as well as the general population at large aware of its presence and scope. Pharmacovigilance should be integrated in undergraduate and postgraduate medical courses